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, quantum dots, gold nanoparticles, or fluorescent dyes, often used in homogeneous immunoassay systems for highly sensitive, wash-free detection.
Key Components in CRET Systems
- Energy Donors (CL Substrates): Luminol is the most common, often used with and a catalyst to produce 3-aminophthalate, which acts as the initial donor. Others include isoluminol and acridinium esters.
- Energy Acceptors: These receive the energy and emit a specific signal.
- Fluorescent Dyes: Fluorescein, TET (6-carboxy-2',4,7,7'-tetrachlorofluorescein), and Rhodamine.
- Nanomaterials: Quantum dots (e.g., CdTe QDs), Graphene Oxide, Gold Nanoparticles (AuNPs), and polymer dots (Pdots).
- Catalysts/Enhancers: Fe(III) deuteroporphyrin IX (FeDP), CuO nanoparticles, or specific short peptides (SPGH) are used to enhance the CL signal, increasing CRET efficiency.
Common CRET Reagent Systems
- Luminol--Quantum Dots: Widely used, where the luminol-system initiates energy transfer to CdTe QDs, commonly used for detecting ions or proteins.
- Luminol-Fluorescein: A classic setup where luminol oxidizes to transfer energy to fluorescein, frequently employed for detecting heavy metal ions.
- Graphene Oxide (GO)-based: Used in 5th-generation chemiluminescence systems, eliminating the need for traditional magnetic bead separation.
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Web results
CRET technique Reagent
Nanjing Poclight Biotechnology Co., Ltd
https://www.poclightbio.com › cret-technique-reagent_sp
Nanjing Poclight Biotechnology Co., Ltd
https://www.poclightbio.com › cret-technique-reagent_sp
This assay is intended for in vitro the quantitative determination of Cardiac Troponin T in human serum and plasma.The test kit is used as an aid in the ...Read more
Chemiluminescence resonance energy transfer
ScienceDirect.com
https://www.sciencedirect.com › article › abs › pii
ScienceDirect.com
https://www.sciencedirect.com › article › abs › pii
by Y Yan2020Cited by 103 — CRET is a non-radiative transfer of energy from chemiluminescence (CL) reagent to energy acceptor during the CL reaction.Read more
Multicolor Chemiluminescent Resonance Energy-Transfer ...
ACS Publications
https://pubs.acs.org › doi
ACS Publications
https://pubs.acs.org › doi
Chemiluminescence resonance energy transfer (CRET) is a non-radiative transfer of energy from chemiluminescence (CL) reagent to energy acceptor during the CL ...Read more
Cerenkov Radiation Energy Transfer (CRET) Imaging - PMC
PubMed Central (PMC) (.gov)
https://pmc.ncbi.nlm.nih.gov › articles › PMC2952622
PubMed Central (PMC) (.gov)
https://pmc.ncbi.nlm.nih.gov › articles › PMC2952622
by RS Dothager2010Cited by 238 — We describe a method for enhancing optical detection of PET isotopes in biological systems, termed Cerenkov radiation energy transfer (CRET).Read more
A strategy to develop new analytical methods
ScienceDirect.com
https://www.sciencedirect.com › article › abs › pii
ScienceDirect.com
https://www.sciencedirect.com › article › abs › pii
by X Huang2012Cited by 87 — Chemiluminescence-resonance-energy transfer (CRET) is non-radiative energy transfer from a chemiluminescent donor to a suitable acceptor molecule.Read more
Focus on CRET technology,Learn about the 5th generation ...
Nanjing Poclight Biotechnology Co., Ltd
https://www.poclightbio.com › blog › focus-on-cret-tech...
Nanjing Poclight Biotechnology Co., Ltd
https://www.poclightbio.com › blog › focus-on-cret-tech...
Aug 8, 2024 — Today we focus on the 5th-generation homogeneous chemiluminescence platform developed by Poclight Biotechnology based on CRET technology.Read more
Schematic representation of CRET process from luminol to ...
ResearchGate
https://www.researchgate.net › figure › Schematic-repre...
ResearchGate
https://www.researchgate.net › figure › Schematic-repre...
a Upon oxidation by H₂O₂ in the presence of catalyst, luminol generates 3-aminophthalate ion, which transfers energy to fluorescein, producing strong CRET ...Read more
Missing: technique Reagent
A Resonance Energy Transfer between Chemiluminescent ...
Wiley Online Library
https://onlinelibrary.wiley.com › doi › anie.200601196
Wiley Online Library
https://onlinelibrary.wiley.com › doi › anie.200601196
Jul 31, 2006 — No need to FRET: An efficient chemiluminescence resonance energy transfer (CRET) between luminol and CdTe quantum dots (QDs) is demonstrated.Read more
Journal of the American Chemical Society
ACS Publications
https://pubs.acs.org › doi
ACS Publications
https://pubs.acs.org › doi
We introduce the CRET-based detection of ATP and DNA by the appropriate modification of QDs with nucleic acids capable of assembling the hemin/G-quadruplex ...Read more
Study of the efficiency of chemiluminescence resonance ...
ScienceDirect.com
https://www.sciencedirect.com › article › abs › pii
ScienceDirect.com
https://www.sciencedirect.com › article › abs › pii
by J Li2022Cited by 7 — The CRET system was based on hemin/G-quadruplex DNAzyme catalyzed luminol analogue L012 and hydrogen peroxide CL system, taking luminol analogue ...Read more