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    AI Overview
    The Veterinary RLN (Relaxin) test using colloidal gold is a rapid, high-sensitivity, visual immunochromatographic assay designed to detect pregnancy in bitches and queens as early as 22-27 days post-breeding. Utilizing serum, plasma, or whole blood, the test acts as a double-antibody sandwich, producing a wine-red line (T) if relaxin is present. 
    Key Aspects of the Veterinary Colloidal Gold RLN Test 
    • Purpose: Detects Canine/Feline Relaxin (cRLN/fRLN), a hormone secreted by the placenta, to confirm pregnancy.
    • Technology: Immunochromatography (lateral flow) using colloidal gold particles for visual identification.
    • Methodology:
      1. Sample (serum/whole blood) is added to the sample well.
      2. If RLN is present, it binds to colloidal gold-labeled monoclonal antibodies on the conjugate pad.
      3. This antigen-antibody complex migrates to the detection line (T), forming a "sandwich" that shows a visible wine-red line.
      4. A control line (C) must appear to validate the test.
    • Advantages: Rapid results (usually within 10-15 minutes), high specificity, user-friendly without need for additional lab equipment.
    • Accuracy: High; can detect pregnancy as early as 22–27 days post-mating. 
    Components of the Test Strip 
    • Sample Pad: Receives the sample.
    • Conjugate Pad: Contains colloidal gold-labeled antibodies.
    • Nitrocellulose Membrane: Contains the Test (T) line and Control (C) line.
    • Absorbent Pad: Maintains consistent liquid flow. 
    • Development of a Test Card Based on Colloidal Gold ... - PMC
      FIG 1. ... Schematic diagram of the colloidal gold test strip and the final product of the colloidal gold test card package. The t...
      PubMed Central (PMC) (.gov)
    • A colloidal gold immunochromatographic test strip based on mAbs ...
      Jun 2, 2025 — The preparation and evaluation of the immunochromatographic strips. If there is a substance to be detected in the sample, it first...
      National Institutes of Health (.gov)
    • Development of a colloidal gold immunochromatographic strip to ...
      Jun 24, 2025 — To overcome these challenges, the colloidal gold immunochromatographic strip has emerged as a widely used detection and analysis m...
      PubMed Central (PMC) (.gov)
    • Pregnancy Relaxin (RLN) Test Kit (Colloidal Gold) - Diagnocine
      The Pregnancy Relaxin test kit can accurately detect if your pet is pregnant as early as 22-27 days post-breeding using a serum or...
      Diagnocine
    • Canine Relaxin (RLN) Test Kit - Babio Biotechnology
      This kit uses double antibody sandwich immunochromatography. If the sample contains sufficient amounts of canine relaxin (RLN), th...
      Babio Biotechnology
    • Pregnancy Relaxin Test Kit (RLN) - A professional supplier of swabs
      Jul 12, 2023 — Test principle of the Pregnancy Relaxin Test Kit This product uses rapid immunochromatography technology to detect the level of re...
      www.chenyanglobal.com
    • Videos about What is Sy-Vgold Veterinary Colloidal Gold ...
      Colloidal Gold Canine Feline Antigen Rapid Test Kit Product Description Colloidal ... Results in 10 Mins Veterinary-Grade Pet Preg...
      Made-in-China.com
    • Canine Relaxin (cRLN) Rapid Test Kit - Flashtest
      Canine Relaxin (cRLN) Rapid Test Kit. This is a colloidal gold detection reagent card used to detect Canine Relaxin (cRLN). Colloi...
      www.flashtestbio.com
    • Development of a Test Card Based on Colloidal Gold ... - PMC
      FIG 1. ... Schematic diagram of the colloidal gold test strip and the final product of the colloidal gold test card package. The t...
      PubMed Central (PMC) (.gov)
    • A colloidal gold immunochromatographic test strip based on mAbs ...
      Jun 2, 2025 — The preparation and evaluation of the immunochromatographic strips. If there is a substance to be detected in the sample, it first...
      National Institutes of Health (.gov)
    • Development of a colloidal gold immunochromatographic strip to ...
      Jun 24, 2025 — To overcome these challenges, the colloidal gold immunochromatographic strip has emerged as a widely used detection and analysis m...
      PubMed Central (PMC) (.gov)
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    My Ad Center

    Web results

    Canine Relaxin (cRLN) Rapid Test Kit


    Flashtest
    https://www.flashtestbio.com › Pets › Rapid Test › Canine
    Flashtest
    https://www.flashtestbio.com › Pets › Rapid Test › Canine
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    Pregnancy Relaxin (RLN) Test Kit (Colloidal Gold)


    Diagnocine
    https://diagnocine.com › Product › Pregnancy-Relaxin-R...
    Diagnocine
    https://diagnocine.com › Product › Pregnancy-Relaxin-R...
    The Pregnancy Relaxin test kit can accurately detect if your pet is pregnant as early as 22-27 days post-breeding using a serum or whole blood sample.Read more
    $13,120.00 · $59.99 delivery · 30-day returns
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    Vet Rapid Test Kit


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    How Is Relaxin Used For Pregnancy Testing In Dogs And ...


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    Colloidal Gold 2 Immunofluorescence Test


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    What is the colloidal gold test used for?
    Colloidal gold technology is widely used in the field of virus detection, which utilizes the specific binding of antigens and antibodies.

    Colloidal gold technology in viral diagnostics - ScienceDirect.com


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    https://www.sciencedirect.com › article › abs › pii
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    How to use colloidal gold rapid screen test?
    Part of a video titled self-diagnostics One Step Pregnancy Test - hCG rapid test - YouTube
    0:57
    2:48
    nal von minden GmbH
    YouTube·
    Apr 11, 2019

    self-diagnostics One Step Pregnancy Test - hCG rapid test

    YouTube·nal von minden GmbH·Apr 11, 2019
    YouTube
    In this video
    "...Minutes. Two red lines the control line and the test line become visible. There is more than a 99% probability that you are pregnant. The two lines may have different color intensities...."
    From 0:57
    Part of a video titled self-diagnostics One Step Pregnancy Test - hCG rapid test - YouTube
    Minutes. Two red lines the control line and the test line become visible. There is more than a 99%MoreMinutes. Two red lines the control line and the test line become visible. There is more than a 99% probability that you are pregnant. The two lines may have different color intensities.

    self-diagnostics One Step Pregnancy Test - hCG rapid test - YouTube


    YouTube
    https://www.youtube.com · nal von minden GmbH
    YouTube
    https://www.youtube.com · nal von minden GmbH
    How accurate is the relaxin test for dogs?
    With a proven accuracy rate of 96%, this easy-to-use home test detects the presence of the relaxin hormone, a reliable indicator of canine pregnancy. No blood draw required. Get fast results in just 10 minutes, allowing you to distinguish between pseudopregnancy and real gestation.

    BELLYLABS Dog Pregnancy Test Kit, Home Use, 96% Accuracy, 10 ...


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    What is the red colloidal gold test?
    Colloidal gold immunochromatographic assay (GICA) is an effective screening method for identifying detectable anti-SARS-CoV-2 neutralizing antibodies.

    Colloidal gold immunochromatographic assay ...


    PMC - NIH
    https://pmc.ncbi.nlm.nih.gov › articles › PMC8180344
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    Web results

    Development of an colloidal gold immunochromatography ...


    Frontiers
    https://www.frontiersin.org › fvets.2025.1626094 › full
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    by J Wang · 2025 · Cited by 2 — We developed a colloidal gold immunochromatographic assay (CGIA) strip using a recombinant BcMSA1-BcSA1 fusion protein, expressed in Escherichia coli with a ...Read more
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